A Metabolomic Investigation of Novel Uremic Biomarkers by Two-Dimensional Liquid Chromatography Mass Spectrometry

摘要

Past identification of novel uremic toxins by mass spectrometry has involved gas chromatography mass spectrometry (GC/MS) and unlike LC/MS or LC/MS/MS is reliant on analyte derivatization to make polar, water soluble analytes amenable for detection. We have developed an online LC-MS/MS methodology capable of isolating and rapidly identifying analytes in hemodialysate with relatively little sample preparation by using accurate mass fragmentation schemes. Novel uremic analytes with the potential to assess dialysis efficiency have been identified and may offer a more suitable alternative to the current biomarkers, urea and creatinine.