Development of a Malignancy-specific Proteome for Non-Hodgkin's Lymphoma

摘要

The dual bromodomain protein Brd2 is closely related to the basal transcription factor TAFII250, which is essential for cyclin A transactivation and mammalian cell cycle progression. In transgenic (Tg) mice, constitutive lymphoid expression of Brd2 causes diffuse large B cell lymphoma (DLCL), which represents most diagnosed human non-Hodgkin's lymphoma (NHL) cases. In an effort to understand the mechanism underlying Brd2-driven B cell lymphoma and how it differs from proliferating or resting B cells, we undertook 2D-PAGE-based comparative proteomic analyses of Tg lymphoma in relation to proliferating and resting B cells from syngeneic, non-lymphoma mice. We hypothesized that, by analogy to our transcriptional profiling results (1), we would deduce a malignancy-specific signature distinct from normal proliferation.