De novo sequencing of porcine Myelin P2 and ligand identification

摘要

This is a complete mass spectrometry based strategy for de novo sequencing of whole proteins. Complementary information provided by CAD and ECD guarantees speed and accuracy without the need for other supplementary techniques like X- Ray crystallography. Moreover, in the present study a rapid and efficient protocol for the extraction and characterization of ligand(s) by GC MS from the crystal has been introduced. This further establishes mass spectrometry as a main stand-alone technique in proteomics and structural analysis.