Method for protein identification from tandem mass spectral employing both spectrum comparison and de novo sequencing for biomedical applications

摘要

A method and algorithm for identifying a protein sequence from mass spectral data combines peptide spectrum matching analysis or spectrum comparison approaches and de novo sequencing approaches. The algorithm of the invention identifies peptide sequences determined independently by each approach, then compares the results and assigns a score reflecting the “goodness” of the match with a full-length protein sequence. Because peptides are identified using independent approaches, the probability of a correct match is increased.