Tandem Mass Tags and MRM Mass Spectrometry for the Evaluation of Biomarkers Implicated in Alzheimer's Disease

摘要

A useful approach for peptide quantitation is combining MRM and peptide labeling (TMT). Using the structurally identical but isotopically different TMT's a reference can be included across validation experiments whilst maintaining the selectivity of MRMs. Excellent correlation between observed and expected ratios for most peptides. For all peptides quantitated 83percent of CVs were <5percent. Rapid transition into biomarker qualification assays from TMT-based discovery studies where LC, MS and MSMS characteristics of proteotypic peptides are already defined. Alleviates the need for immunoassay assay development and the production of heavy isotope-labeled peptides. Future experiments will focus on the validation of candidate biomarkers implicated in AD.