Global Metabolite Profiling of Human Urine using LC-MS on a new Ion-Trap Time of Flight hybrid Mass Spectrometer.

摘要

Global metabolite profiling is emerging as a powerful tool in a number of research and development fields including biomarker discovery, disease diagnosis and drug efficacy. NMR was first applied to profiling studies, principally as a result of strong quantitative data, however, MS provides higher sensitivity and has led to a greater acceptance of this technology in exploring profiling research. With the development robust ion source performance together with high mass accuracy and resolution, LC-MS has been applied to large sets of real samples (biological fluids, extracts etc) and enables subsequent statistical analysis by multivariate statistics to identify key biomarkers and patterns of disease. This paper describes the application of high mass accuracy MSn analysis applied to human urine profiling studies.