Enzymatic glycosylation of bakuchiol and vanillin in Supercritical carbon dioxide media

摘要

Enzymatic syntheses of water soluble bioactive compounds as their glycosides were carried out under supercritical CO_2 (SCCO_2) conditions for vanillin, bakuchiol and retinol. With Bakuchiol, under SCCO_2 (10 M Pa pressure at 50°C), bakuchiol formed glycosides with D-glucose, D-galactose, D-mannose, D-fructose, D-ribose, D-arabinose, D-sorbitol and D-mannitol in yield range 9% to 46.6%. Antioxidant and ACE inhibitory activity of bakuchiol glucosides showed that 6-O-(6-D-Fructofruranosyl) bakuchiol showed the best antioxidant (1.4mM) and ACE inhibitory activities (0.64 mM). Further vanillin glycoside formed in corresponding yield of 56% with glucose in SCCO_2 media.