EFFECT OF STEM APICAL CULTURE ON BIOLOGICAL NITROGEN FIXATION ABILITY OF SUGARCANE

摘要

Aims: To investigate the effect of tissue culture on the endophytic nitrogen fixation ability of the plants. Methods and results: Sugarcane stem apical culture was conducted with introduced varieties B1, B3, B5 and B8 from Brazil and domestic varieties GT11 and ROC16. The plants derived from stem apical culture that had been cloned for 4 generations were treated with and without nitrogen. The other experiment was conducted by pot culture without nitrogen fertilizer in the greenhouse. Under no nitrogen condition, the nitrogen fixation capacity and growing vitality of the Brazilian varieties B1, B3, B5 and B8 were stronger than those of GT11 and ROC16, and the Brazilian varieties had stronger growing capacity, higher nitrogen content and higher nitrogenase activity, and their symptom of nitrogen deficiency appeared later. In the pot cultivation experiment, the Brazilian varieties showed more stalks, stronger tillering and nitrogenase activity in leaves than GT11 and ROC16. The results indicated thatthe regenerated plants of the tested sugarcane varieties did not lose the biological nitrogen fixation ability after stem apical culture. Significant and Study: The results provided effective reference for introduction and propagation of sugarcane varieties and germplasm with nitrogen fixing capacity.