MOLECULAR GENETIC DIVERSITY OF SUGARCANE GENOTYPES OF SUBTROPICAL INDIA: SSCP-PCR ANALYSIS OF SIMPLE SEQUENCE REPEATS

摘要

THE POTENTIAL of Single Strand Conformational Polymorphism (SSCP) of simple sequence repeats as a new marker system was investigated with respect to their abundance, variability and ability to detect polymorphisms and molecular genetic diversity in sugarcane genotypes. Eleven microsatellite markers (derived from genomic libraries) were used as primers to amplify genomic DNA from 42 commercial cultivars, interspecific hybrids and species of sugarcane. Amplified SSR-PCR products were subjected to SSCPto detect polymorphism to determine genetic diversity and inferring relationship of the studied genotypes. The resulting patterns ranged from few bands to highly complex multi-band ladders for most of the primers. SSCP-PCR demonstrated a high level of polymorphism between the studied genotypes. The presence or absence of individual amplified product DNA bands was scored to derive a pair-wise distance matrix between genotypes and their clustering in different groups. Thus, the SSCP-PCR analysis of simplesequence repeats is useful to differentiate even closely related taxa. The major advantage of this method is that many individual PCR products may be screened for variation simultaneously. This is the first report of the use of SSCP-PCR to differentiatebetween members of a highly polyploid genus.