Allele frequencies of 15 STR loci in an Italian population

摘要

STRs are today one of the most effective tools for individual and populational genetic characterization This paper presents the results of a population study of 15 STR loci included in AmpFlSTR Identifiler kit (Applied Biosystems) that has the arm of creating a local database. Blood or oral swab samples were obtained from 100 unrelated individuals, who were born in Terni (in the center of Italy) and lived there for at least two generations. DNA was extracted from bloo,using QIAamp, miniKit of Qiagen and from oral swa,using Chelex method For each locus allele frequency was calculated and Hardy-Weinberg equilibrium was evaluated. The Expected Heterozygosity (He), the Observed Heterozygosity (Ho), the Polymorphic Information Content (PIC), the Power of Discrimination (PD) and the Power of exclusion (PE) were also calculated.