A single assay for human-specific quantification ofless than 1 pg DNA and detection of the presence ofPCR inhibitors in forensic samples

摘要

We describe the development, validation, and application of a duplex quantitative realtime PCR assay fbi human-specific quantification of DNA samples containing as little as 0.5 pg/mul of DNA with the presence of an internal positive control (IPC) allowing simultaneous detection of PCR inhibitors The combination of human DNA quantity and quality data provides the forensic DNA analyst with substantial information prior to STR amplification, thereby reducing the number of samples needing re-evaluation following initial profiling The features of this assay will allow us to apply it very effectively to evaluation of touch evidence and low copy number samples.