Special tissue sampler was developed, allowing measuring concentration of photosensitizer in various biopsy materials with high accuracy. The method is based on simultaneous measurement of fluorescence and reflectance spectra and biopsy of tissue sample embedded in a spherical aperture by a diameter of 1.5 mm and a depth of 1 mm. Both surfaces of the sampler were covered with an impenetrable black film. Meterological measurements were done on standard solutions consisting solutions "Photoscence" and "Intralipid" in various ratios. We used a fiber optic spectrometer LESA-01-BIOSPEC to measure optical properties of the solution with various concentrations of "Intralipid" and "Photoscence." Results follow theory. To measure concentration of photosensitizer in micro volumes and thin layers, special multifiber catheters with external diameter from 0.5 mm to 1.8 mm were prepared.
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