TOTAL INTERNAL REFLECTION-BASED BIOCHIP FOR HIGH THROUGHPUT BIOASSAYS

摘要

The construction of a total internal reflection-based biochip utilizing an integrated micromirror array is presented. The evanescent field that dramatically increases the signal to noise ratio, is generated through the implementation of a four-layer chip, containing silicon micromirrors and UV curable polymer cavities integrated on top of a microfluidic network. The design enables the compact, vertical integration of a laser diode and a CCD camera, eliminating the need to precisely align the laser beam into the system. The multilayer chip can be used as an optical-microfluidic platform for various multiplexed bioassays or as miniaturized component for an integrated handheld lab-on-a-chip microsystem.