Release of Ferulic Acid and Reducing Sugars from De-starched Wheat Bran by Using Mixed enzymes of Ferulic acid esterase and Arabinoxylanase

摘要

Mixed enzymes containing ferulic acid esterase (FAE) and carbohydrases, mainly arabi-noxylanase (AX) prepared from lipid culture of Aspergillus niger were used to hydrolyze de-starched wheat bran. The results showed that the optimal reaction pH and temperature for the enzymes were 4.4 and 50°C respectively, however, no significant effect of metal ions tested on the activity of enzymes was observed. Hydrolysis of de-starched wheat bran with the enzymes by three times released 75.46% of soluble substances from de-starched wheat bran.