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Semen extension: a comparison of methods

机译:精液扩展:方法比较

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摘要

Many boar studs currently pre-extend semen with the thought of reducing cold shock. The most important factors influencing sperm function include collection temperature, storage temperature, and the suspension medium. When spermatozoa are cooled too abruptly from body temperature to less than 15 degrees C cold shock can occur, which may reduce sperm viability. Semen should be extended as soon as possible after collection from the boar. Pre-extension is also thought to provide a more gradual osmotic change using a small amount (100ml) of extender and following up with full extension. Pre-extending semen also quickly exposes the raw ejaculate to antibiotics. Bacteria are a normal component of the boar ejaculate, and have been reported to range in concentrations of up to 10~9 cfu/ml. Pre-extension, however is time consuming in the boar stud. The possibility of full extension in a single step would save time and the potential for error since the semen is handled once instead of two separate times. Theaim of this study was to determine if there was a significant difference in reproductive performance between sows that were inseminated with pre-extended semen compared to those that were inseminated with semen that was not pre-extended.
机译:许多野猪螺柱目前预先延长了精液,以思想降低冷休克。影响精子功能的最重要因素包括收集温度,储存温度和悬浮料。当精子从体温突然冷却至小于15摄氏度时,可能会发生冷休克,这可能会降低精子存活率。从野猪收集后,应尽快延长精液。预扩展也被认为使用少量(100ml)的扩展器和完全延伸提供更逐渐的渗透变化。预延伸精液也很快促使原料射精到抗生素。细菌是猪射精的正常组分,并据报道,浓度为高达10〜9 cfu / ml的范围。然而,预延伸,在野猪螺柱中是耗时的。自精液被处理一次而不是两个单独的时间以来,单步中完全扩展的可能性会节省时间和错误可能性。本研究的胫骨是确定是否在与未预先延长的精液专生的母猪之间产生的母猪之间的生殖性能存在显着差异。

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