The composition for dog semen freezing, the dog semen frozen method of using this and the artificial insemination method of using this frozen dog semen

摘要

(57) Abstract This invention the composition for freezing dog semen, is the frozen method of using this and something regarding artificial insemination. This invention, per distilled water 100ml, the sodium citrate 0.25 - 4.5g, dextrose 0.45 - 4.0g, penicillin 0.01 - 0.3g, streptomycin 50,000 - 500,000 IU, the catalase 0.25 - 0.45g, the yolk 0.045 - 0.25g, the glycerine 1.5 - 15ml and the serum of the dog offers the composition for dog semen freezing which contains 0.02 - 0.1g. Furthermore, this invention the stage which extracts the semen of the male dog (a) due to massage method or electric shocking method and, (b) the description above centrifugal separating the semen which was picked, does the fluid of the higher stratum of society section, in order the semen separated stage which takes only the lower stratum and, (for the semen of c) aforementioned lower stratum and capacity ratio the aforementioned composition with to be 1:1, mixes, furthermore adds the aforementioned composition to the blend which is formed the stage which makes refrigerate primarily and, (d) the description above (by c) stage in order for the aforementioned semen and capacity ratio the composition with to be 1:2, mixesIt does, it offers the frozen method of the dog semen which includes with the stage which makes the blend freeze which is formed the stage which makes refrigerate secondarily and, (e) the description above (d) by stage including liquid nitrogen.