TYPING OF PRRSV ISOLATES IN THAILAND BY A NESTED MULTIPLEX PCR

摘要

Porcine reproductive and respiratory syndrome virus (PRRSV) is a member of the arterivirus first observed in the United States (US) in 1987 and in Europe (EU) in 1990 (1). A serological survey of swine sera during 1988-1999 in Thailand demonstrated the earliest detection of seropositive animals in 1989 and the percentage of seropositive animals increased annually from 8.6 in 1991 to over 79 in 1999 (4 ). However, PRRSV was first isolated in Thailand from suckling and nursery pigs with severe chronicrespiratory distress and the virus was identified as the US serotype (2). Since Thailand has continuously imported swine breeders from both European and North American countries for genetic improvement of breeding stocks, both EU and US serotypes may bepresence in the swine population in Thailand. The objective of this study was to isolate and determine the serotypes of the PRRSV recently isolated from clinical samples in Thailand using a multiplex PCR.