MAJOR VIRULENCE FACTOR DETECTION AND VEROCYTOTOXIN TYPE 2 SUBTYPE FROM CLINICAL E. COLI ISOLATES USING A ONE-STEP MULTIPLEX PCR

摘要

A single kit comprising 3 multiplex PCR assays that can detect in E. coli thepresence of the 8 virulence genes: eaeA, EHEC-HlyA, Stx1 (VT1), Stx2(VT2),Stx2c (VT2c), Stx2d (VT2d), Stx2e (VT2e) and Stx2f (VT2f) is described.In addition, the kit can detect the two critical serotypes (O157 and H7) andidentify the species (Escherichia coli) simultaneously using a one stepreaction. Following evaluation in our hands, this PCR kit has been used todetect the above 11 components of disease-causing E. coli in a fast, accurate,reliable and specific fashion. These kits can be used on bacterial isolatesand has the potential for use directly on foods and environmental samples.