Toward Improved Biochips Based on Rolling Circle Amplification—Influences of the Microenvironment on the Fluorescence Properties of Labeled DNA Oligonucleotides

摘要

Microarrays have become an increasingly important tool for biotechnology and molecular diag-nostics. Despite many advantages, their sensitivity is still insufficient for such tasks as the analysisof small sample quantities and for the detection of alterations in gene expression of low-abundancegenes. Accordingly, amplification strategies are necessary. Approaches to amplify the signal inten-sity include the increase of the number of dye molecules per target through either particle labelsor rolling circle amplification, as used for this study.