Field trials, the permitting process, comments and risks

摘要

We detail experiences in the process of designing and preparing experiments, obtaining permits and the perceived and potential risks associated with trial releases of genetically modified/transformed arthropods. What was first proposed as a free release was modified to an environmental release in very large field cages. The insects to be released were transformed with a piggyBac transposable element encoding the fluorescent protein EGFP. Biological characteristics of the transformed strain are detailed. Descriptions and responses to the comments and criticisms received during the public commentary and comment period associated with obtaining the release permit are detailed and analyzed. Certain of the critiques provided during the permitting process are summarized and discussed, as are our responses to those critiques. Discussion is made of some perceived hazards and estimates of risk associated with such a release along with a discussion of statistical analysis.