Fish Detects Cytogenetics Abnormalities in Almost All CLL Patients with Progressive

摘要

We tested a set of commercially available probes to determine the feasibility and accuracy of FISH in the assesment of abnormalities in Chronic Lymphocyryic Leukemia (CLL) patients. We utilized 3 different probes for the 13ql4.3 region, one for chromosome-12-specific alpha- satellite DNA probe; one for the P53 gene at 17pl3.1, and one for 3'-5'IgH at 14q32 covering the entire region of IgH, thus potentially allowing to detect more rearrangements. Results from 13 patients studied showed a normal karyotype in 8 patients. FISH was able to detect chromosomal abnormalities in 10 (85%): +12 in 4 (38%), range 47-58% of positive nuclei; del 13q in 4 (38%), range 32-88%; del of P53 in 3 (38%),range 34-68%; del of 5 IgH in 1 (15%), in 60% of the nuclei. Interestingly one patient showed both deletion of P53 and for all the probes covering the 13ql4 region. One of 3 patients with a del 13ql4, showed a deletion of LSI D13S319 and D13S25 probes (DBM region) while RB-1 stayed at 13ql4. Even if in a small group of patients, FISH confirmed its ability to improve the detection of cytoge-netic abnormalities, and in particular this strategy seems to be promising for future studies.