Screening of Variables in β-Xylosidase Recovery Using Cetyl Trimethyl Ammonium Bromide Reversed Micelles

摘要

β-Xylosidase recovery by micelles using cetyl trimethyl ammonium bromide (CTAB) cationic surfactant was verified under different experimental conditions. A 2~(5-1) fractional factorial design with center points was employed to verify the influence of the following factors on enzyme extraction: pH (x_1), CTAB concentration (x_2), electrical conductivity (x_3), hexanol concentration (x_4), and butanol concentration (x_5). Statistical analysis of the results shows that of the five variables studied only hexanol and electrical conductivity did not have significant effects on the recovery of β-xylosidase. The other factors had significant effects in increasing order: (x_1) > (x_2) > (x_5). The model predicts a recovery value of about 45%, which is similar to that obtained experimentally (43.5%).