Subunit interactions of chloroplast ATP synthase from higher plant

摘要

The chloroplast ATP synthase is made up of nine subunits and is physically and functionally composed of two parts, CF_o and CF_1. The chloroplast F_oF_1 most closely resembles the bacterial enzyme in its subunit composition, overall structure and amino acid sequence homology of some subunits. But the chloroplast ATP synthase is unique among the ATP synthases in several respects (Richter et al. 2000). In this study, we detected the subunit interactions among the chloroplast ATP synthase subunits using the yeast two-hybrid system, and the important roles of interaction between gamma and epsilon subunits in the functions of enzyme were also examined by mutagenesis analysis.