Rice oxalate oxidase interacts with protein kinase in yeast two-hybrid system

摘要

The SNF1 protein kinase from yeast is conserved in eukaryotes. SNF_1 gene encodes a ser/thr protein kinase required for expression of glucose-repressible genes in response to glucose deprivation. The mammalian homologue of SNF_1, AMP-activated protein kinase (AMPK) is involved in cellular stress response. SNF_1 homologues have been cloned from Arabidopsis, rice, barley, potato, rye, tobacco and sugar beet. Both SNF_1 and AMPK #alpha# protein kinase interact with regulatory #beta# and #gamma# sub-units to form active heterotrimeric complexes. In plants, #beta# and #gamma# sub-units interacting with SNF_1-like protein kinase have been identified in Arabidopsis and potato. OSK_3 protein belongs to SNF_1-like protein kinase, which is strongly expressed in rice immature seed. Some evidences suggest that SNF_1-like protein kinases play an important role in regulating basic metabolic pathways in response to nutritional an environmental stress. It was reported that osk_3 gene was induced after treatment by fungal elicitor derived from Magnaporthe grisea. In this report, we present several candidate proteins interacting with OSK_3 protein kinase by using yeast two-hybrid system.