Study of stability of expression of a humanized monoclonal antibody from a transfected NSO myeloma cell line using different culture media and serum concentrations

摘要

The use of mammalian cell lines for expression of recombinant proteins with potential therapeutic use has become increasingly prevalent. Optimising productivity by recombinant cells relies partly on understanding relationships between regulation of protein expression and culture conditions, e.g,, composition of cell culture medium. Stability of expression of recombinant cell lines is a major problem for many researchers and manufacturers.