Optimization of Medium for Bacillus thuringiensis Recombinant with CCRD

摘要

The medium was employed and divided into two factors for further optimization with shaking cultivation on the basis of the previously related research. the relationship between component factors (x_i) of the medium and spore numbers (y) of Bacillus thuringiensis (Bt) recombinant was demonstrated with the central composite rotable design (CCRD). A response surface equation was formed as follows: y=308.5-28.34_(x_1) + 3.17_(x_2) - 30.25_(x_1~2) - 61.25_(x_2~2) + 46_(x_1x_2) and then the best combination of medium, ∑C_1 = 0.75%, ∑C_2 = 0.44% and ∑C_3 = 0.1%, was rapidly acquired.