SUPPRESSIVE EFFECT OF FUCOIDAN DERIVED FROM MOZUKU ON IN VITRO INVASION OF HUMAN FIBROSARCOMA HT1080 CELLS

摘要

Fucoidan is a uniquely-structured sulfated polysaccharides that is found in the cell walls of several types of brown seaweed, but not of land plants. Recently, Fucoidan has clinically got a lot attention due to its anti-tumor metastasis potential. Wereport here that the enzyme-digested fucoidan extract derived from Cladosiphon novae-caledoniae kylin, which was called "mozuku" in the Kingdom of Tonga, inhibited in vitro invasion of human fibrosarcoma HT1080 cells. Flow cytometric analysis revealed that the enzyme-digested fucoidan extract effectively scavenged intracellular H_2O_2 of HT1080 cells. Both activities of MMP2 and 9 were decreased in a dose dependent manner by the fucoidan extract, suggesting that Fucoidan may inhibit the invasion of tumor cells via suppression of MMP2 and 9 activities.