To enhance enzymatic hydrolysis of straw, various pretreatments, including mechanical pertreatment and removal of non-cellulose componets with chemical or biological pretreatment, was evaluated. The results indicated that ball milling as a pretreatment substantially reduced crystallinity of substrate and enhanced to following enzymatic hydrolysis rate when the moisture in the substrate was controled. The lignin content in substrated was one of most important factors effected the enzymatic hydrolysis, but removal of 50
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