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High-Resolution Three-Dimensional Imaging with an X-Ray Microscope

机译:具有X射线显微镜的高分辨率三维成像

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The Gottingen transmission X-ray microscope (TXM) at BESSY has been used for threedimensional imaging. As a first test-object sheaths of the bacteria Leptothrix Ochracea have been visualized. 33 tilted-view images have been taken at tilt angles between 0 and 160 degrees in steps of 5 degrees. Before reconstruction the images were flat-field corrected and aligned. The rotation axis was determined and the images were rotated accordingly. For tomographic reconstruction a fast MART (Multiplicative Algebraic Reconstruction Technique) algorithm was used. In the recon-structed volume structures of sizes well below 100 nm are made visible.
机译:Bessy的Gottingen传输X射线显微镜(TXM)已用于三维成像。作为细菌的第一个测试对象鞘,已经可视化。 33倾斜视图图像在0到160度的步长为5度的倾斜角度。在重建之前,图像校正并对齐。确定旋转轴并且相应地旋转图像。对于断层切断重建,使用了快速的MART(乘法代数重建技术)算法。在尺寸的尺寸尺寸的结构中,使得可见尺寸远低于100nm。

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