Patterning of biomolecules on a biocompatible nonchemically amplified resist

摘要

A simple lithographic process in conjunction with a novel biocompatible nonchemically amplified photoresist material was successfully used for the patterning of biomolecules such as cells and proteins. UV light irradiation on selected regions of the nonchemically amplified resist film renders the exposed regions hydrophilic by the formation of carboxylic groups. Mouse fibroblast cells were found to be preferentially aligned and proliferated on the UV light exposed regions of the nonchemically amplified resist film, where carboxylic groups were present. For streptavidin patterning, amine-terminated biotin was linked to the carboxylic groups of the UV light exposed regions, which was further used to bind streptavidin to the UV light exposed regions.