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Influence of hematoporphyrin on the mechanical properties of the lipid bilayer membranes

机译:血卟啉对脂质双层膜力学性能的影响

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The interactions between hematoporphyrin (HP) and artificial bilayer lipid membranes (BLM) has been studied. No influence of HP on the BLM conductivity has been observed at the HP concentrations from 10$+$MIN@6$/ to 3 $MUL 10$+$MIN@5$/ M. The modulus of elasticity in the direction normal to the membrane plane (E$-$PRP$/) and the dynamic viscosity coefficient ($eta) have been measured, both exhibiting the HP- induced decrease by 22 $DIV 31% in darkness. Under illumination by low- intensity He-Ne laser ($lambda $EQ 632 nm) the values of parameters E$- $PRP$/ and $eta of the Hp-modified membranes were found to increase by 41 $DIV 66%. When removing the HP from the solution by perfusion, the irreversible changes in mechanical properties of the HP-modified membranes caused by the laser light were observed. This could be due to formation of the stable complexes of HP with lipid molecules.
机译:已经研究了血液卟啉(HP)和人造双层脂质膜(BLM)之间的相互作用。 HP浓度从10 $ + $最小的HP浓度@ 6 $ /至3 $ MUL 10 $ + $ MIN @ 5 $ /米的惠普浓度没有影响HP对BLM电导率的影响。在正常的方向上弹性模量已经测量了膜飞机(e $ prp $ /)和动态粘度系数($ eta),两者都在黑暗中表现出惠普引起的22美元的31%。在低强度HE-NE激光($ Lambda $ EQ 632 NM)下的照明中,发现惠普改性膜的参数值的价值观为41美元66%。当通过灌注从溶液中除去HP时,观察由激光引起的HP改性膜的机械性能的不可逆变化。这可能是由于形成具有脂质分子的HP的稳定复合物。

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