Fluorescence spectroscopy has been extensively investigated for disease diagnosis. In this framework, optical tissuephantoms are widely used for validating the biomedical device system in laboratory environment outside clinicalprocedures. Moreover, it is fundamental to consider that there are several scattering components and chromophoresinside biological tissues and the interplay between scattering effects and absorption can result in a distortion of theemitted fluorescence signal. In this work, the photophysical behaviour of a set of liquid tissue like phantoms containingdifferent compositions was analysed: phosphate buffer saline (PBS) was used as background medium, low fat milk as ascatterer, India ink as an absorber and PpIX dissolved in dimethyl formamide (DMF) as fluorophore. We examined thecollected data in terms of the impact of surfactant Tween-20 on the background medium, scattering effects andcombination of scattering and absorption within a luminescent body on PpIX. The results indicated that the intrinsicemission peaks are red-shifted by the scattering particles or surfactant, whilst the scatterer and absorber can alter theemission intensity substantially. We corroborated that phantoms containing higher surfactant content (> 0.5% Tween20) are essential to prepare a stable aqueous phantoms.
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