The single-beam gradient force optical tweezers have transformed various elds of scientic research by enablingmanipulation and characterization of single molecules. Conventional optical tweezers pose limitations in trap-ping particles in the sub-Rayleigh regime. These limitations have been overcome with the help of plasmonicnanoapertures like the double-nanohole aperture. A modied colloidal lithography technique has been used infabrication of double-nanohole apertures achieving dimensions appropriate for trapping single molecules in thisregime. This paper demonstrates optical trapping of a single 10 nm enzyme, rubisco, using double-nanoholeapertures fabricated using the modied colloidal lithography technique as well as presents the results from trans-mission characterization of dierent double-nanohole apertures carried out using the nite-dierence time-domain(FDTD) simulations.
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