Molecular Characterization and Expression of Ribosomal Protein L15 Gene (RPL15) From Arachis hypogaea

摘要

RPL15 is a component of the large ribosomal subunit 60S. The sequences of cDNA and DNA of RPL15 were cloned successfully from Arachis hypogaea cultivar Ri Hua 1. These two sequences were analyzed preliminarily. The full length RPL15 cDNA of A. hypogaea contains a 615-bp open reading frame (ORF) which encodes 204 amino acids. The length of RPL15 gene was 1383 bp with three exons and two introns. Primary structure analysis revealed that the molecular weight of the putative RPL15 protein is 24211.09 Da with a theoretical pI of 11.44. The deduced amino acid sequence of RPL15 (A. hypogaea cultivar Ri Hua 1) shared homology with other reported species. The expression of RPL15 transcript showed that RPL15 mRNA was expressed mainly in root. The cDNA of RPL15 was cloned successfully from A. hypogaea in this study. It provides scientific material for enriching and improving the RPL15 gene database.