Optimization of culture conditions for improving the biomass of Agaricus brunnescens

摘要

Agaricus brunnescens is a producer for novel biomaterial (exopolysaccharide of Agaricus brunnescens).In this paper,the optimization of culture conditions for the biomass of Agaricus brunnescens through orthogonal methods was reported.The optimal conditions for enhancing production was:inoculum size 10 ％,broth volume 100 mL in a 250 mL flask,pH 6.0.This optimization strategy in shake flask culture lead to an biomass 0.8 g/L,which was considerably higher than that obtained in preliminary studied.