Both Phases indicate seeding density to be a significant factor in resultant construct properties. Phase 2 indicated that growth factors supplementation during cell expansion may be used to improve construct properties following self-assembly. By developing mechanically robust neocartilage, this work illustrates the potential for the clinical translation of self-assembled CC cells for replacing damaged TMJ cartilages. It is demonstrated that TFP may be used in conjunction with aggregate redifferentiation and the self-assembly process to develop tissues with mechanical properties nearing native tissue values. Furthermore, it is demonstrated that a smaller initial cell population may be used to develop such tissues, illustrating the potential for autologous tissue replacements.
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