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Extraction and Evaluation on Anti-fatigue Function of Polysaccharide from Auricularia auricula

机译:黑木耳多糖的提取及其抗疲劳作用的评价

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The best solution to the polysaccharide ultrasonic extraction of auricularia is as follows. With aqueous conditions of solid-liquid ratio of 1:60, 4h extraction time, 40w ultrasonic power, 70°C extraction temperature, extract twice to measure the auricularia polysaccharide extraction rate as 14.95%. Then elute the auricularia polysaccharide with DEAE-Cellulose 52 chromatographic column to get auricularia polysaccharide component AAP -1 which is then separated by Sephadex G-200 gel chromatographic column to get mono component. Set up the swimming training model with increasing intensity. Feed the mouse witsh AAP-1 solution. The result shows that a certain dose of AAP-1 taken can increase the contents of muscle glycogen and liver glycogen and maintain a constant blood sugar. The exhaustion time of the mouse can be elongated and the survival time in anoxic condition will be significantly prolonged. The above shows that auricularia polysaccharide has the function of anti-exercise fatigue.
机译:乌耳多糖超声波提取的最佳方法如下。在固液比为1:60,提取时间为4h,超声功率为40w,提取温度为70°C的水溶液条件下,提取两次,测出耳桐多糖的提取率为14.95%。然后用DEAE-纤维素52色谱柱洗脱木耳多糖,得到木耳多糖组分AAP -1,然后用Sephadex G-200凝胶色谱柱分离得到木耳多糖组分,得到单组分。建立强度越来越大的游泳训练模型。用AAP-1解决方案喂鼠标。结果表明,一定剂量的AAP-1可以增加肌肉糖原和肝糖原的含量,并保持恒定的血糖。小鼠的疲劳时间可以延长,缺氧条件下的存活时间将大大延长。由上可知,木耳多糖具有抗运动疲劳的功能。

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