Production of Diacylglycerols Using Lipase-Catalyzed Esterification and Glycerolysis

摘要

Diacylglycerols(DAG) was synthesized through esterification of caprylic acid andglycerol, as well as glycerolysis of medium-chain triacylglycerols(GTCC), catalyzed by lipaseNovozym 435 (from Candida Antarctica), respectively. A comparative study of the esterificationwas conducted in a batch reactor and a packed bed reactor, respectively. The effect of reactionparameters, such as solvent, temperature, molar ratio of the reactants, water removal, enzymeloading and ratio of height to diameter of the reactor on the conversion of caprylic acid and theDAG content in the reaction mixture were studied. The conversion of caprylic acid and the contentof DAG esterification in the packed bed reactor reached 82.30% and 77.26%, respectively, by using200U /g caprylic acid at a space velocity of 34h~(-1), 60°C in 7h; while the esterification in the batchreactor present a caprylic acid conversion of 83.91% and a DAG content of 68.24% with 50U /gcaprylic acid at 60°C in 10h. The glycerolysis in t-butanol yielded 26.26% of monoacylglycerols(MAG), 36.02% of DAG, with a GTCC conversion of 60% under the optimum conditions.