Application of Agilent 2100 Bioanalyzer in Multiplex PCR for Detection of Foodborne Pathogens

摘要

To explore the feasibility of using Agilent 2100 Bioanalyzer in the detection of the three kinds of food-borne pathogens, three pairs of primers were designed according to invA gene of Salmonella ipah gene of Shigella and inv gene of Yersinia pseudotuberculosis, and then the single PCR was performed to detect specificity. Those genes were amplified by Multiplex PCR technique using specific primers, and the optimum of multiplex PCR reaction conditions. The multiplex PCR detection method which provided a basis for further research on developing the food-borne pathogens detection kit was very practical. Agilent 2100 Bioanalyzer combined with Multiplex PCR was specific, accurate and sensitive for foodborne pathogens Salmonella, Shigella and Yersinia pseudotuberculosis detection.