Aqueous extraction and alkaline (NaOH) hydrolysis method were comparatively studied in polysaccharides extraction from submerged-cultured Agaricus blazei mycelia. The results show that the polysaccharide content and the total extract rate from alkaline extraction method were obviously higher than those from the aqueous extraction method. Optimization results by the orthogonal matrix method on polysaccharide extraction from A. blazei show that the optimum alkaline concentration was 1.0 mol/L, hydrolysis temperature 60°C, hydrolysis time 1 hour, and liquid: solid ratio 15. With the optimized condition, the polysaccharide extraction rate reached 11.93 ±± 0.68 %. The mycelial polysaccharide obtained with alkaline hydrolysis method was fractionated by the DEAE column chromatography. Five APS fractions were isolated in the polysaccharide sample with APS-1 as the main component
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