Molecular cloning and characterization of a vacuolar H+ -pyrophosphatase gene from the xerophyte Zygophyllum xanthoxylum

摘要

Drought and salinity are the two severe abiotic stresses result in reduction of output of agriculture worldwide.Increased vacuolar Na+ compartmentalization and vacuolar solute accumulation can confer plants salt-and drought-tolerance. Overexpression of vacuolar H+-pyrophosphatase ( VP) gene can potentiate the active transport of Na+ into vacuole by establishing the H+ electrochemical potential difference between the vacuole lumen and the cell cytosol (Gaxiola et al.,2001).A homologous gene (ZxV P1‐1) of vacuolar H+ -pyrophosphatase was cloned and characterized from an endemic genera plant Zygophyllum xanthoxylum, which lives in the desert area of middle Asia with distinctive mechanism on drought resistance.