Movement measurement of isolated skeletal muscle using imaging microscopy

摘要

Abstract: An imaging-microscopy methodology to measure contraction movement in chemically stimulated crustacean skeletal muscle, whose movement speed is about 0.02 mm/s is presented. For this, a CCD camera coupled to a microscope and a high speed digital image acquisition system, allowing us to capture 960 images per second are used. The images are digitally processed in a PC and displayed in a video monitor. A maximal field of 0.198 $MUL 0.198 mm$+2$/ and a spatial resolution of 3.5 $mu@m are obtained. !6