Different sensitivity of cells from tumor-bearing organisms to continuous-wave and pulsed laser radiation (lambda = 632.8 nm) evaluated by chemiluminescence test

摘要

Abstract: Chemiluminescence test results were used to evaluate the sensitivity of human blood and murine splenocytes to continuous-wave (CW) and pulsed He-Ne laser light. It is demonstrated that CW radiation has in our experimental conditions practically no effect on the luminol-amplified chemiluminescence of four models under study. The pulsed radiation had a week inhibiting effect on the samples from healthy organisms but inhibited markedly the chemiluminescence of samples from tumor-bearing organisms. The effect depended on duration of dark period between pulses. A transient local heating mechanism is proposed to explain the inhibition of activity of NADPH-oxidase. !18