Through above experimental research, in pectinase method, with the change of enzymolysis temperature, pH, dosage of enzyme and enzymatic hydrolysis time, the extraction yield of chlorogenic acid in honeysuckle was changing. The optimal condition was that solid-liquid ratio was 1:50 (g/mL), enzyme hydrolysis temperature was 50 °C, enzymatic hydrolysis pH was 5, dosage of enzyme was 0.5%, enzymatic hydrolysis time was 120 min, and the extraction yield of the chlorogenic acid was 4.75%. In the ultrasonic extraction method, the optimal condition was ultrasonic temperature was 40 °C, ultrasonic power was 280 W, ultrasonic time was 80 min, ethanol concentration was 70%, ultrasonic solid-liquid ratio was 1:30 (g/mL), and the extraction yield of chlorogenic acid reached 6.26%.
展开▼
