The Effect of Collagen Vitrigel Nanostructure on Human ESC-Derived Retinal Pigment Epithelial Maturation

摘要

In this study, we evaluated the ES-denved RPE maturation on different substrates, including synthetic polymer and collagen-based materials. RPE cells proliferate well on different substrates and became mostly pigmented and polarized between 4-6 weeks. Quantitative study by qPCR showed RPE characteristic marker expression significantly increased in CV3D group. This study provided an insight of how nanofibril in collagen based material influence RPE maturation.