Characterization of In-vitro Release of Gentamicin from Biodegradable Polymer Thin Films Microstructure-Function Relationship by Confocal Raman Microscopy

摘要

The release kinetics of gentamicin sulfate from thin polymer films could be tailored to meet the desired release profile. Among other factors, gentamicin sulfate particle size seems to have the most pronounced effect on the initial burst release of drug. The mechanism of burst release was further confirmed using CRM. Tools like CRM that can detect and quantify spatial distribution of drug in similar formulation are critical in developing models that describe the kinetics of drug release, linking drug distribution on the microscopic scale to macroscopic performance of the product. This technique offers distinct advantages in comparison to other analytical techniques like imaging by light and electron microscopy that lack chemical information and require preparatory procedures that can introduce artifacts. With minimal sample preparation, both qualitative and quantitative analysis at ～0.3μm spatial resolution can be achieved. Future efforts will seek to improve the accuracy of the models to predict the API particle size distribution and efforts are underway to quantify spatial distribution by quantitative chemical imaging.