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Primary Characterization and In Vitro Antioxidant Activities of Polysaccharides from Yam Peel

机译:山剥离的多糖的主要表征和体外抗氧化活性

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The research purpose is to make full use of yam peel resources. In this present study, yam peel polysaccharide (YPP) was isolated from the Dioscorea esculenta peels. The primary characterization of YPP was explored, and we carried on the in vitro antioxidant experiment research. The FT-IR analysis was performed. And the absorptions at around 1,148 cm~(-1) indicated a pyranose form of sugars. In vitro, YPP exhibited a potent scavenging activity on the hydroxyl radical, DPPH radical and superoxide radical, especially scavenging activity for DPPH radicals. The IC_(50) value of YPP for eliminating hydroxyl radicals, DPPH radical, and superoxide anion radicals was respectively 9.5, 2.1, and 10.2 mg/ml. And the highest inhibition rates were, respectively, 60.2, 79.3, and 54.0 %. In this experiment, antioxidation test in vitro indicated that YPP was similar to other yam tuber polysaccharides. Furthermore, the further works on purification and isolation studies were done and the primary characterization of YPP were shown by chro-matography. In general, these results suggested that the YPP may be explored as a novel and effective natural antioxidant to alleviate oxidative stress, and they may find promising applications as potent antioxidant agents and antiaging medicines.
机译:研究目的是充分利用山药剥离资源。在本研究中,从Dioscorea eSculenta剥离中分离山药剥离多糖(YPP)。探讨了YPP的主要表征,我们进行了体外抗氧化实验研究。进行FT-IR分析。并且存在约1,148cm〜(-1)的吸收表明吡喃糖形式的糖。体外,YPP在羟基自由基,DPPH激进和超氧化物中表现出有效的清除活性,特别是DPPH自由基的清除活性。用于消除羟基自由基,DPPH自由基和超氧化物阴离子的yPP的IC_(50)值分别为9.5,2.1和10.2mg / ml。最高的抑制率分别为60.2,79.3和54.0%。在该实验中,体外抗氧化试验表明YPP类似于其他山药块状多糖。此外,完成了对纯化和分离研究的进一步作品,并通过Chro-Matography示出了YPP的主要表征。通常,这些结果表明,ypp可以被探索为一种新颖且有效的天然抗氧化,以减轻氧化应激,并且它们可以找到有效的应用作为有效的抗氧化剂和抗衰析药物。

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