Engineering sequence and stimuli dependent doxorubicin release from anti-nucleolin aptamer coated gold nanoparticles

摘要

A state-of-art drug loading/release system based on anti-cancer doxorubicin (DOX) intercalation into anti-cancer oligonucleotide, coated on ~ 4nm gold nanoparticles (GNP) has been developed. This anticancer hybrid system was developed by covalently functionalizing GNPs with an anti-cancer thiolated-oligonucleotide (AS1411) and/or its control (CRO) sequence, followed by incubation with DOX under dark and ambient conditions. The 1:1 coating of AS1411: CRO on GNPs efficiently releases DOX by dithiothreitol (DTT) reduction of oligonucleotide-thiol linkage or by y--radiation exposure. Size and stability of GNP Oligonucleotides were investigated with dynamic light scattering (DLS) and zeta potential characterizations respectively. DOX loading/release in GNP Oligonucleotides was investigated using fluorescence studies.