Preparation and Evaluation of Fish Scale Peptide/β-TCP Composites as Bone-Repairing Materials

摘要

This study related to the preparation of FSP/β-TCP scaffolds (tube and microsphere) by reacting fish scale peptide (FSP) with β-tricalcium phosphate (β-TCP) and 1-ethyl-3-(3-dimethyl aminopropyl) carbodimide (EDC) by 1: cross-linking reaction (defined chemical cross-linking); 2: repeated frozen and freeze drying (defined physical cross-linking). The results showed it was hard to produce a stabilized tube or microsphere under physical cross-linking treatment, due to the low molecular weight of fish scale peptide (M. w about 3000). However, addition of alginate as an extra component and cross-linked by CaCl_2, it could strengthen and stabilize the shape and be able to produce FSP/β-TCP microsphere (or tube). These microspheres had good spherical shape, smooth surface morphology and different mechanical strengths.