Preparation of peptides hydrolyzed from rape pollen glutelins

摘要

Rape pollen stored more than 1 year was used as material in this study. A research on protein fractionation of rape pollen defatted and cell-fragmentated was performed. Rape pollen gluntelin was hydrolyzed with alcalase and the crude peptides of rape pollen were obtained. The scavenging effect of rape pollen glutelin and its hydrolysate on hydroxyl free radicals(·OH) in the deoxyribose-iron system was studied. The results showed that glutelins and albumins were found to be the predominant proteins in rape pollen,comprising 55.7% and 39.0% of total proteins, While globulins and prolamins were 3.2% and 2.1%,respectively.The optimum conditions of alcalase enzymatic hydrolysis have been determined by mono-factor analysis and response surface methodology as follows:pH =9,hydrolyzing temperature 50°C,enzyme concentration 1460U per gram of substrate, concertration of substrate 6%,hydrolyzing time 2h. In the antioxidation test, the inhibition rate ·OH of glutelins was 25.1%. After being hydrolyzed, the inhibition ratio of·OH of glutelins peptide was singnificently increased to 70.0%.